The tumorigenicity of immortalized cells differentiated from mouse embryonic stem cells.
- Author:
Gan SHEN
1
;
Xiao-Qian CONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinogenicity Tests; Cell Differentiation; Cells, Cultured; Embryonic Stem Cells; cytology; Endothelial Cells; cytology; Endothelium, Vascular; cytology; Mice; Mice, Inbred Strains; Mice, Nude; Telomerase; genetics; Transfection
- From: Chinese Journal of Plastic Surgery 2012;28(1):33-39
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo discuss the tumorigenicity of immortalized endothelial cells differentiated from embryonic stem cells.
METHODSThe embryoid bodies (EB) formed in vitro from embryonic stem cells, were induced to differentiate into many "round cells" (the precursor of endothelial cells). These "round cells" later formed the vascular tube-like structures. To immortalize these cells, human telomerase reverse transcriptase (hTERT) cDNA was transfected into "round cells" by lipofectin, RT-PCR and immunocytochemistry were used to evaluate the immortalized cells. And the tumorigenicity of these cells were evaluated by being injected into nude mice subcutaneously.
RESULTS95% of these transfected cells expressed Flk-1, CD34 and vWF, and could proliferate in large quantity in vitro (cell number was doubled in 2 days, and increased 12 times in 3 days), and were able to form tubular structures.
CONCLUSIONSThese results suggest that hTERT cDNA transfection can immortalize induced endothelial cells and tumorigenicity is found after immortalized cells are injected into nude mice subcutaneously.
