Influence of MicroRNA-382 on Biological Properties of Human Umbilical Cord-Derived Mesenchymal Stem Cells.

Jun-jie Cui; Ying Chi; Xin Yang; Yu-yan Shen; Zhao Wang; Su-dong Zhang; Li-ning Zhang; Li Liu; Shi-hong LU; Ming-zhe Han; Si-zhou Feng

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Influence of MicroRNA-382 on Biological Properties of Human Umbilical Cord-Derived Mesenchymal Stem Cells.

Author: Jun-Jie CUI ¹ ; Ying CHI ¹ ; Xin YANG ¹ ; Yu-Yan SHEN ¹ ; Zhao WANG ¹ ; Su-Dong ZHANG ¹ ; Li-Ning ZHANG ¹ ; Li LIU ¹ ; Shi-Hong LU ¹ ; Ming-Zhe HAN ¹ ; Si-Zhou FENG ²
Author Information

1. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
2. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. E-mail: sizhoufeng@medmail.com.cn.
Publication Type:Journal Article
MeSH: Cell Differentiation; Core Binding Factor Alpha 1 Subunit; metabolism; Granulocyte Colony-Stimulating Factor; metabolism; Granulocyte-Macrophage Colony-Stimulating Factor; metabolism; Humans; Indoleamine-Pyrrole 2,3,-Dioxygenase; metabolism; Interleukin-6; metabolism; Macrophage Colony-Stimulating Factor; metabolism; Mesenchymal Stromal Cells; cytology; MicroRNAs; metabolism; Osteogenesis; Transfection; Umbilical Cord; cytology
From: Journal of Experimental Hematology 2016;24(3):852-857
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the effect of microRNA-382 (miR-382) on the biological properties of human umbilical cord-derived mesenchymal stem cells (hUC-MSC).
METHODSThe mimics and inhibitor of miR-382 were transfected into hUC-MSC with lipo2000. Inverted microscopy was used to observe the morphology change of hUC-MSC. The proliferation of hUC-MSC was detected by CCK-8. Oil red O and alizarin red staining were applied to assess the adipogenic and osteogenic differentiation of hUC-MSC. Cetylpyridinium chloride was used to the quantitative analysis of osteogenic differentiation. The expression of Runx2 and some cytokines were detected by RT-PCR.
RESULTSmiR-382 did not influence the morphology, proliferation and adipogenic differentiation of hUC-MSC miR-382 inhibited the expression of Runx2, thus could inhibit the osteogenesis of hUC-MSC, being confirmed by alizarin red stain; miR-382 could influence the expression of key cytokines secreted from hUC-MSC, such as IL-6, IDO1, G-CSF, M-CSF, GM-CSF.
CONCLUSIONmiR-382 decreases the expression of Runx2 and inhibites the osteogenesis of hUC-MSC. In addition, it also affects the expression of some key cytokines secreted from hUC-MSC.