Differentiation of K562 Cells Induced by Pulsatilla Saponin A into Erythroid Lineage.

Tong Wang; Ri Zhang; Xiao-fei QI; Sha-sha Bai; Fang Gong; Zi-xing Chen; Jian-nong Cen; Ming-qing Zhu; Lan Dai

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Differentiation of K562 Cells Induced by Pulsatilla Saponin A into Erythroid Lineage.

Author: Tong WANG ¹ ; Ri ZHANG ¹ ; Xiao-Fei QI ² ; Sha-Sha BAI ¹ ; Fang GONG ¹ ; Zi-Xing CHEN ¹ ; Jian-Nong CEN ¹ ; Ming-Qing ZHU ¹ ; Lan DAI ¹
Author Information

1. Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province,China.
2. Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province,China. E-mail：qixf-sz@hotmail.com.
Publication Type:Journal Article
MeSH: Antineoplastic Agents; Cell Differentiation; Cell Lineage; Erythroid Cells; Humans; K562 Cells; Saponins
From: Journal of Experimental Hematology 2016;24(4):975-979
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore the differentiation-inducing potentiality of Pulsatilla saponin A on K562 cells.
METHODSPulsatilla saponin A of different concentrations was used to treat K562 cells; the benzidine staining and the hemoglobinometry were applied to measure the change of hemoglobin content; the flow cytometry (FCM) was used to detect the expression of CD71 and GPA on K562 cells.
RESULTSK562 cells treated with 4 µg/ml pulsatilla saponin A differentiated into the erythroid lineage. With the treatment of pulsatilla saponin A, the hemoglobin content in K562 cells increased significantly; CD71 and GPA expression on the K562 cell surface were up-regulated.
CONCLUSIONPulsatilla saponin A can induce K562 cells to differentiate into erythroid lineage.