Effect of Icaritin on K562 Cell Proliferation and Intracellular Reactive Oxygen Species.
- Author:
Ting MA
1
;
Zi-Jian LI
1
;
Li-Na WANG
1
;
Zhuo LI
1
;
Xiao-Li MU
1
;
Ying LYU
1
;
Ya-Ming XI
2
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Proliferation; Extracellular Space; Flavonoids; Humans; K562 Cells; Reactive Oxygen Species
- From: Journal of Experimental Hematology 2016;24(4):1003-1007
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of icaritin (ICT) on proliferation of K562 cells and reactive oxygen species (ROS) in patients with chronic myeloid leukemia (CML).
METHODSMTT assay was used to detect the effect of ICT on the proliferation of K562 cells. Flow cytometry was used to detect the apoptosis of K562 cells and intracellular ROS level. The expression of PARP protein was detected by Western blot.
RESULTSICT obviously inhibited the proliferation of K562 cells and induced their apoptosis, the expression of PARP protein was enhanced, and the intracellular ROS increased significantly.
CONCLUSIONThe ICT showes the inhibitory effects on proliferation and apoptosis-inducing effects on K562 cells, and thier mechanism relats with the increase of reactive oxygen species in the cells.
