Action Mechanism of Chlorambucil against Mantle Cell Lymphoma Cell Line Jeko-1.
- Author:
Jian-Xin GUO
1
;
Ya-Hong ZHOU
2
;
Jing-Xin PAN
1
;
Xi-Zhe GUO
1
;
Shi-Xin WU
1
;
Yue-Qin HUANG
1
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Cell Proliferation; Chlorambucil; Down-Regulation; Humans; Lymphoma, Mantle-Cell; Phosphatidylinositol 3-Kinases; Phosphorylation; Signal Transduction
- From: Journal of Experimental Hematology 2016;24(4):1066-1070
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the action mechanism of chlorambucil against mantle cell lymphoma cell line Jeko-1.
METHODSThe effect of chlorambucil on Jeko-1 cell proliferation was measured by MTT method. The effect of chlorambucil on the apoptosis of Jeko-1 cell was detected by Hoechst staining and Annexin V-FITC dual staining. The activation of PI3K/AKT signaling pathway and the expression of BAX, BCL-2, procaspase 3, procaspase 8 and procaspase 9 were detected by Western blot.
RESULTS0, 5, 10, 20 µmol/L chlorambucil could inhibit Jeko-1 cell proliferation at 24, 48, 72 h in a time- and dose-dependent manner. Chlorambucil of 0, 5, 10, 20 µmol/L increased the apoptotic rate of Jeko-1 cells, upregulated the expression of BAX, procaspase 3, procaspase 8, procaspase 9 and PI3K, increased the phosphorylation of AKT and down-regulated the expression of BCL-2.
CONCLUSIONThe chlorambucil can induce the apoptosis of mantle cell lymphoma Jeko-1 cells via blocking PI3K/AKT signaling pathway.
