Role of miR137-MITF in Prognostic Analysis of Multiple Myeloma.

Shuai-shuai Zhang; Yan XU; Shu-hui Deng; Chang-hong LI; Wen Zhou; Lu-gui Qiu

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Role of miR137-MITF in Prognostic Analysis of Multiple Myeloma.

Author: Shuai-Shuai ZHANG ¹ ; Yan XU ¹ ; Shu-Hui DENG ¹ ; Chang-Hong LI ¹ ; Wen ZHOU ¹ ; Lu-Gui QIU ²
Author Information

1. State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300020,China.
2. State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300020,China. E-mail：drqiu99@medmail.com.cn.
Publication Type:Journal Article
MeSH: Cell Line, Tumor; Humans; MicroRNAs; Microphthalmia-Associated Transcription Factor; Multiple Myeloma; Prognosis
From: Journal of Experimental Hematology 2016;24(4):1096-1099
CountryChina
Language:Chinese
Abstract:
UNLABELLEDObjectiive：To explore the effect of miR137 target gene MITF on the prognosis of multiple myeloma (MM).
METHODSThe target genes of miR137 were predicted by software, the GFP analysis was carried out for detecting MITF as the prognosis of multiple myeloma. The cell line overexpressing miR137 in MM cell line was constructed. Real-time qPCR and Western blot were used to detect the expression of MITF in this cell line.
RESULTSThe target genes of miR137 were MITF, BUE2H, SH3BP5 and KLF12. High expression of MITF in MM patients showed a good prognosis according to GFP analysis, but no significant difference was detected between the different subgroups. MITF expression was higher in MM cell line that over expressed miR137.
CONCLUSIONThe miR137-MITF is an important index in judging the prognosis of multiple myeloma.