Dendritic Cells Promote the Proliferation of Peripheral Blood CRTH2 Cells (CD4(+)CD294(+)Th2) and Help B Cells to Secrete Immunoglobulin.
10.7534/j.issn.1009-2137.2016.04.037
- Author:
Fa-Qing TIAN
1
,
2
;
Juan LI
3
;
Ju-Heng LI
4
;
Mei-Qin TANG
4
;
Xiao-Hui CHENG
4
;
Ying-Cai HUANG
5
;
Hui-Qing LI
5
Author Information
1. Department of Hematology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China
2. Department of Hematology, Longgang District People's Hospital of Shenzhen, Shenzhen 518172, Guangdong Province, China.
3. Department of Hematology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China.
4. Department of Hematology, Longgang District People's Hospital of Shenzhen, Shenzhen 518172, Guangdong Province, China.
5. Blood Cell Laboratory, Longgang District People's Hospital of Shenzhen, Shenzhen 518172, Guangdong Province, China.
- Publication Type:Journal Article
- MeSH:
B-Lymphocytes;
Cell Proliferation;
Coculture Techniques;
Dendritic Cells;
Flow Cytometry;
Humans;
Immunoglobulins;
Lymphocyte Culture Test, Mixed;
Th2 Cells
- From:
Journal of Experimental Hematology
2016;24(4):1163-1167
- CountryChina
- Language:Chinese
-
Abstract:
Objective：To investigate the promotive effect of dendritic cells(DCs) on proliferation of CRTH2 (CD4(+)CD294(+)Th2) cells and the influence of CRTH2 cells on secretion of immunoglobulin from B cells so as to provide a new approach for amplification and sorting of Th2 cells. Methods：DCs were induced from peripheral blood mononuclear cells, then the loaded-BCGV-Ag-DCs were cocultured with T cells, and the mixed lymphocyte reaction(MLR) was performed by CCK8 method. The phenotypes of DCs and CRTH2 cells were detected by flow cytometry. CRTH2 cells sorted by MACS were co-cultured with B cells for 5 days to detect the secretion of immunoglobulin. Results：The subsets and absolute number CRTH2 cells were significantly increased by loaded-BCGV-Ag-DCs. The levels of IgG, IgA and IgE were higher increased in supernatant of CRTH2 and B cell co-culture system than that in control group or that in transwell group(P<0.05). Conclusion：The proliferation of CRTH2 cells can be greatly promoted by loaded-BCGV-Ag-DCs, and the CRTH2 cells can help B cells to secrete IgG, IgA and IgE.
