Influence of S1PR5 Defect on the Lymphocyte Distribution in Mice.
- Author:
Zhen-Yang GU
1
;
Xiao-Li ZHAO
1
;
Nan YAN
1
;
Li WANG
1
;
Fei-Yang WANG
1
;
Li-Li WANG
1
;
Chun-Ji GAO
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow; Cell Count; Flow Cytometry; Lymphocytes; Lysophospholipids; Mice; Receptors, Lysosphingolipid; Sphingosine; analogs & derivatives
- From: Journal of Experimental Hematology 2016;24(4):1168-1172
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDThe sphingosine 1-phosphate (S1P) receptors (S1PRs) are a group of G protein-coupled receptors expressed on the surface of lymphocytes. The interaction between S1P and S1PRs plays a significant role in the migration and distribution of lymphocytes.
OBJECTIVETo investigate the influence of S1PR5 defect on the lymphocytes distribution in mice.
METHODSThe distribution of different subsets of lymphocyte in the mice with S1PR5 defect was examined by flow cytometry.
RESULTSCompared with wild type mice, the number of NK cells in the peripheral blood (PB) and spleen (SP) from the mice with S1PR5 defect decreased very significantly 〔PB: 6.4±0.45% vs 2.2±0.47(P<0.01,n=3)；SP: 3.0±0.91% vs 0.68±0.14%(P<0.05,n=3)〕. However, the NK cell number in the bone marrow (BM) and lymphonodes (LN) of the mice with S1PR5 defect increased very significantly 〔BM: 0.97±0.20 % vs 2.6±0.35% (P<0.01, n=3); LN: 0.35±0.16% vs 1.7±0.15% (P<0.01, n=3)〕. The percentages of CD3(+) lymphocyte in peripheral blood, spleen and lymph node were not statistically significantly different between these 2 types of mice 〔PB: 17.3±7.9% vs 17.0±4.6% (P>0.05, n=3); SP: 33.0±6.0% vs 27.4±1.8% (P>0.05, n=3); LN: 42.3±10.7% vs 51.2±2.7% (P>0.05, n=3)〕.
CONCLUSIONS1PR5 defect can significantly influence the NK cell distribution.
