Bone morphogenetic protein signal transduction pathway regulates runx2 expression in MC3T3 -E1 osteoblasts in vitro induced by centrifugation.
- Author:
Jian GUAN
1
;
Zong-sheng CHENG
;
Jian-ping WANG
;
De-chao LI
;
Hui-xin DENG
Author Information
- Publication Type:Journal Article
- MeSH: Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins; Cell Differentiation; Centrifugation; Core Binding Factor Alpha 1 Subunit; Gene Expression Regulation; Humans; In Vitro Techniques; Osteoblasts; RNA, Messenger; Signal Transduction
- From: West China Journal of Stomatology 2010;28(1):38-44
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the expression of Runx2 in osteoblasts in response to centrifugation in vitro and discuss the function of bone morphogenetic protein (BMP) signal transduction pathway in this course.
METHODSCells were divided into four groups, group A, B, C and D, pretreated with DMEM containing 10% fetal bovine serum, 10% fetal bovine serum, 100 ng x mL(-1) Noggin and 100 ng x mL(-1) Noggin for 24 hours separately. 271 x g centrifugation was loaded for 5 min to these groups except group A and C, other conditions were the same. The total RNA of each group were extracted, and reversed transcription to cDNA after 30 min. The expression of Runx2 in response to centrifugation in vitro was analyzed by quantitative real time PCR.
RESULTSThe expression of Runx2 mRNA in group B was significantly higher than that in group A (P < 0.05). The expression of Runx2 mRNA in group D was significantly lower than that in group B (P < 0.05). There was no statistically significant difference among group A, C, D (P = 0.692).
CONCLUSIONBMP signal transduction pathway plays an important role in the response of osteoblasts to mechanical stimulations. It may also play a central role in the cascade information dissemination of osteoblasts.
