Study of signal transduction pathway in the expression of inflammatory factors stimulated by lipopolysaccharides from Porphyromonas endodontalis in osteoblasts.
- Author:
Di YANG
1
;
Li-hong QIU
;
Ren LI
;
Zi-mu LI
;
Chen LI
Author Information
- Publication Type:Journal Article
- MeSH: Humans; Imidazoles; Interleukin-6; Lipopolysaccharides; MAP Kinase Signaling System; Osteoblasts; Porphyromonas endodontalis; Pyridines; RNA, Messenger; Signal Transduction; p38 Mitogen-Activated Protein Kinases
- From: West China Journal of Stomatology 2010;28(2):135-138
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo quantify the interleukin (IL)-1beta mRNA and IL-6 mRNA expression induced by lipopolysaccharides ([PS) extracted from Porphyromonoas endodontalis (P. endodontalis) in osteoblasts, and to relate P. endodontalis LPS to the bone resorptive pathogenesis in the lesions of chronic apical periodontitis.
METHODSMG63 cells was pretreated with PD98059 or SB203580 for 1 h and then treated with P. endodontolis LPS for 6 h. The expression of IL-1beta mRNA and IL-6 mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR) technique.
RESULTSThe production of IL-1beta mRNA induced by P. endodontalis LPS decreased in osteoblasts pretreated with PD98059. Both of the production of IL-1beta mRNA and JL-6 mRNA induced by P. endodontalis LPS decreased in osteoblasts pretreated with SB203580.
CONCLUSIONThe synthesis of IL-1beta mRNA stimulated by Pendodontalis LPS in MG63 probably occur via extracellular signal-regulated kinase (ERK) 1/2 and p38 mitogen activated protein kinase (MAPK) signal transduction system. The synthesis of IL-6 mRNA stimulated by P.endodontalis LPS in MG63 probahly occur via p38MAPK signal transduction system.
