Experimental study on application recombinant human bone morphogenetic protein 2(rhBMP-2)/poly-lactide-co-glycolic acid (PLGA)/fibrin sealant(FS) on repair of rabbit radial bone defect.
- Author:
Zhongkai FAN
1
;
Yang CAO
;
Zhe ZHANG
;
Mingchao ZHANG
;
Wei LU
;
Lei TANG
;
Qi YAO
;
Gang LU
Author Information
1. Department of Orthopaedics, First Affiliated Hospital, Liaoning Medical University, Jinzhou 121000, China. flanz@sohu.com
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Morphogenetic Protein 2;
therapeutic use;
Bone Regeneration;
drug effects;
Bone Substitutes;
therapeutic use;
Female;
Fibrin Tissue Adhesive;
therapeutic use;
Lactic Acid;
therapeutic use;
Male;
Microspheres;
Polyglycolic Acid;
therapeutic use;
Rabbits;
Radius Fractures;
therapy;
Recombinant Proteins;
therapeutic use;
Transforming Growth Factor beta;
therapeutic use
- From:
Journal of Biomedical Engineering
2012;29(5):903-907
- CountryChina
- Language:Chinese
-
Abstract:
This paper is aimed to investigate the repair of rabbit radial bone defect by the recombinant human bone morphogenetic protein 2/poly-lactideco-glycolic acid microsphere with fibrin sealant (rhBMP-2/PLGA/FS). The radial bone defect models were prepared using New Zealand white rabbits, which were randomly divided into 3 groups, experiment group which were injected with eMP-2/PLGA/FS at bone defect location, control group which were injected with FS at bone defect location, and blank control group without treatment. The ability of repairing bone defect was evaluated with X-ray radiograph. Bone mineral density in the defect regions was analysed using the level of ossification. The osteogenetic ability of repairing bone defect, the degradation of the material, the morphologic change and the bone formation were assessed by HE staining and Masson staining. The result showed that rhBMP-2/PLGA/FS had overwhelming superiority in the osteogenetic ability and quality of bone defect over the control group, and it could promote the repair of bone defect and could especially repair the radial bone defect of rabbit well. It may be a promising and efficient synthetic bone graft.
