Recombinant OspC identification and antigenicity detection from Borrelia burgdorferi PD91 in China.
- Author:
Jian CHEN
1
;
Kang-Lin WAN
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Bacterial; Bacterial Outer Membrane Proteins; genetics; immunology; Blotting, Western; Borrelia burgdorferi Group; immunology; Escherichia coli; genetics; Humans; Lyme Disease; diagnosis; Polymerase Chain Reaction; Recombinant Proteins; analysis; immunology
- From: Chinese Journal of Epidemiology 2003;24(10):917-919
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo recombine OspC gene from Borrelia burgdorferi PD91 of China and expressed it in E. coli for early diagnosis of Lyme disease.
METHODSThe OspC gene was amplified from the genome of Borrelia burgdorferi PD91 strain by polymerase chain reaction and recombined with plasmid PET-11D. The recombinant plasmid PET-11D-OspC was identified with PCR, restriction endonuclease analysis and sequencing. The antigenicity was verified with Western Blot.
RESULTSOspC gene was cloned correctly into vector PET-11D. The resultant sequence was definitely different from the published sequence. The recombinant OspC seemed to have had strong antigenicity.
CONCLUSIONThe findings laid basis for the studies on early diagnosis of Lyme disease.
