Preliminary study on the association of chemokine RANTES gene polymorphisms with HIV-1 infection in Chinese Han population.
- Author:
Xue-Lin LIU
1
;
Fu-Sheng WANG
;
Lei JIN
;
Ming-Xu LIU
;
De-Zhong XU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Case-Control Studies; Chemokine CCL5; genetics; metabolism; Child; China; Female; Gene Frequency; Genetic Predisposition to Disease; genetics; Genotype; HIV Infections; genetics; HIV-1; Haplotypes; Humans; Introns; genetics; Male; Middle Aged; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; genetics; Sequence Analysis, DNA; Sex Factors
- From: Chinese Journal of Epidemiology 2003;24(11):971-975
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the single nucleotide polymorphism (SNP) of chemokine regulated upon activation, normal T cell expressed and secreted (RANTES) promoter and first intron of asymptomatic, human immunodeficiency virus 1 (HIV-1) infected individuals of in Han Chinese and evaluate the influence on HIV-1 infection by variants.
METHODSCase-control study was adopted, Genotypes of RANTES promoter -403 and -28 from 538 samples and RANTES first intron In1.1 from 300 samples of Han Chinese were detected by DNA sequencing or by polymerase chain reaction-restriction fragment length polymorphism.
RESULTSThere were six genotypes of RANTES promoter -403 and -28 found in Han Chinese group. The distribution of genotypes was AC/AC 12.4%, AC/AG 3.5%, AC/GC 29.2%, AG/GC 10.9%, GC/GC 42.1%, AG/AG 1.5%. The haplotypes was GC 62.7%, AC 28.7%, AG 8.6%. Compared with AC/AC, Odd ratio (OR) of RANTES genotypes AC/AG, AC/GC, AG/GC, GC/GC was associated with weaker reduced susceptibility to HIV-1 infection. However, there were no significant contents of the allele frequencies between people living with HIV-1 and healthy individuals. The distribution of RANTES In1.1 alleles were T/T 71.0%, C/T 19.9%, C/C 9.1% and haplotypes were RANTES In1.1T 81%, In1.1C 19%, respectively; There were significant differences of RANTES In1.1 between people with HIV-1 infection and healthy individuals in males. The In1.1C-bearing genotypes would increase susceptibility to HIV-1 infection but no significant differences in females were found. Strong linkage disequilibrium was observed between all of the three RANTES SNPs.
CONCLUSIONThe two -403A/G, -28C/G variants in RANTES promoter region and intron In1.1 T/C mutation genotype were found to be associated with the genetic susceptibility to HIV-1 infection among the Han Chinese. However, the In1.1C allele or its haplotypes in RANTES intron 1 displayed a stronger dominant association with HIV-1 infection in males.