A molecular epidemiological study on the human immunodeficiency virus infection in Jiangsu province.
- Author:
Hai-tao YANG
1
;
Hui XING
;
Cheng-mei JIA
;
Hao LIANG
;
Xiao-qin XU
;
Ming WEI
;
Lei LI
;
Zhao CHEN
;
Yi-ming SHAO
Author Information
- Publication Type:Journal Article
- MeSH: China; epidemiology; Genes, env; genetics; HIV Infections; blood; epidemiology; HIV Seroprevalence; HIV-1; classification; genetics; Humans; Polymerase Chain Reaction; Sequence Analysis, DNA
- From: Chinese Journal of Epidemiology 2003;24(11):976-979
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify subtypes of human immunodeficiency virus 1 (HIV-1) strains and their distribution, infection sources, and the trends of HIV infection in Jiangsu province.
METHODSAnticongulated bloods from 46 infected persons were collected to separate previrus DNA. HIV-1 env genes were then amplified by nested-PCR and sequenced for their C2-V3 region so as to identify subtypes. The analysis of consensus sequence, genetic distance and phylogenetic tree were conducted with GCG software.
RESULTSBy the end of 2001, there had been six subtypes of HIV-1 strains identified in Jiangsu province: A, B, B', C, D and E. The predominant subtypes were C (accounting for 40.48%) and B' (accounting for 38.10%). Subtype C accounted for 86.67% among injecting DUs while subtype B' accounted for 91.67% among commercial blood donors and receivers.
CONCLUSIONSubtype B'among commercial blood donors was brought to Jiangsu from neighboring provinces. The outbreak of HIV-1 infection among local DUs was caused by subtype C from Xinjiang province. Findings from HIV/AIDS molecular epidemiologic study suggest that it is challenging for Jiangsu to treat patients, apply vaccine, prevent and control AIDS in the future.
