Genetic analysis of Yersinia pestis strains isolated in China.

Author: Dong-zheng YU ¹ ; Rong HAI ; Xing-qi DONG ; Min LI ; Lian-xu XIA ; Xian-ming SHI ; Jian-chun WEI ; Bai-zhong CUI ; Peng WANG ; Lian-zhi SUN ; Zhi-kai ZHANG ; Yuan HU ; En-min ZHANG
Author Information

1. Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Publication Type:Journal Article
MeSH: China; Databases, Genetic; Electrophoresis, Gel, Pulsed-Field; Genetic Drift; Geography; Mutation; Random Amplified Polymorphic DNA Technique; Yersinia pestis; classification; genetics; isolation & purification
From:Chinese Journal of Epidemiology 2003;24(11):1005-1009
CountryChina
Language:Chinese
Abstract:
OBJECTIVEThe strains of Yersinia pestis isolated in different period and different natural foci in China were analyzed.
METHODSTraditional and molecular biological methods were used. Rhamnose fermentation, rRNA gene copy number, nitrite reduction, and the glycerol fermentation were important characters for typing, and pulse field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) profile could reflect the genetic distance between the strains.
RESULTSThe strains could be divided into 15 genetic types by those 6 characters with each of them covered an isolated geographical territories.
CONCLUSIONThe characters of strains were described; the genetic relationship of different types, their evolution, and the forming and shift of plague natural foci were analyzed.