Surveillance finding on rotavirus in Changchun children's hospital during July 1998-June 2001.
- Author:
Li-wei SUN
1
;
Zhi-li TONG
;
Li-hong LI
;
Jing ZHANG
;
Qi CHEN
;
Li-shu ZHENG
;
Jing LIU
;
Hua-ping XIE
;
Cheng-xun WANG
;
Li-jie ZHANG
;
B IVANOFF
;
R I GLASS
;
J S BRESEE
;
X I JIANG
;
P E KILGORE
;
Zhao-yin FANG
Author Information
- Publication Type:Journal Article
- MeSH: Child, Preschool; China; epidemiology; Diarrhea; etiology; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Feces; virology; Female; Genotype; Hospitals, Pediatric; statistics & numerical data; Humans; Infant; Male; Reverse Transcriptase Polymerase Chain Reaction; Rotavirus; classification; genetics; isolation & purification; Rotavirus Infections; complications; epidemiology; virology; Sentinel Surveillance; Serotyping
- From: Chinese Journal of Epidemiology 2003;24(11):1010-1012
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish baseline patterns of rotavirus diarrhea and to describe its epidemiologic features in Changchun city, prior to rotavirus vaccine immunization.
METHODSHospital-based surveillance was conducted among children under 5 years old with acute diarrhea in Changchun Children's Hospital. Fecal samples were determined to identify rotavirus by PAGE and/or ELISA. G serotypes of rotavirus were identified by ELISA and/or nested RT-PCR. P genotyping were carried out by RT-PCR. All data were computerized and analysed by "Generic Manual on Rotavirus Surveillance" set by CDC in the USA.
RESULTSIn total, 2 343 diarrhea cases were screened and 1 211 fecal samples were collected. Rotavirus was detected in 31.0% among outpatients and 52.9% in inpatients. During the peak of the season (November through March), 58.6% of diarrhea was caused by rotavirus among inpatients. 95.0% of rotavirus diarrhea cases occurred among children aged < 2 years. The predominant strain was serotype G1 (82.4%), followed by G2 (5.0%), G3 (3.3%), G4 (0.9%). P genotyping showed that P[8] and P[4] were the most common ones. Nine different P-G combinations were identified, four strains (P[8]G1, P[4]G2, P[8]G3, and P[8]G4) commonly seen worldwide accounted for 75.6% of the total. Taken together with uncommon strains, including the novel types P[4]G4 and P[8]G2, it highlights the extraordinary diversity of rotaviruses circulating in China.
CONCLUSIONRotavirus is the major cause of severe child diarrhea in Changchun. Developing a rotavirus vaccine for prevention of severe disease and reduction of treatment costs seemed to be necessary.