A new type of spotted fever group Rickttsiaes detected in the area of Changbai mountain, Jilin province.
- Author:
Yong-jian HAO
1
;
Wu-chun CAO
;
Shu-ping GAO
;
Pan-he ZHANG
;
Qiu-min ZHAO
;
Hong YANG
;
Xi-tan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Outer Membrane Proteins; genetics; China; DNA, Bacterial; chemistry; genetics; Humans; Phylogeny; Polymerase Chain Reaction; Rickettsia Infections; microbiology; Rickettsieae; classification; genetics; Sequence Analysis, DNA
- From:Chinese Journal of Epidemiology 2003;24(12):1126-1128
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn order to find out the current situation of tick-borne spotted fever in the area of Changbai mountain, Jilin province.
METHODSIn this study, a polymerase chain reaction (PCR) method was developed with primers R. rOmpA 190.70p and R. rOmpA 190.701n designed on the basis of rOmpA gene, which is specific for examining spotted fever group Rickttsiaes (SFGR). Six hundred nighty-three ticks were tested and a positive PCR product amplified from D. silvarum specimen (named JL-02) was cloned and sequenced.
RESULTSThe SFGR DNA was detected from D. silvarum, Haemaphysalis concinna with the positive rates were 53.81% and 7.41% respectively. Its nucleotide sequence of 587 bp rOmpA and derived amino-acids showed 100.00% similarity with nucleotide sequence of DnS 14 and 99.00% with DnS 28 from the Former Soviet Union according to the result of BLUST and CLUSTAL, which was differential from the DNA sequences of strains previously detected in China.
CONCLUSIONThe natural focus of tick-borne spotted fever did exist in the area of Changbai mountain. The DNA sequence of SFGR was similar to that of DnS 14, which was first reported in China.
