Study on ribotyping of Lyme borreliosis spirochete in Guizhou province.
- Author:
Ding-ming WANG
1
;
Qin HAO
;
Xing-he CAI
;
Kang-lin WAN
;
Zhao-xiao WANG
;
Jian CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Borrelia burgdorferi; classification; genetics; China; DNA, Bacterial; chemistry; genetics; DNA, Ribosomal Spacer; genetics; Humans; Lyme Disease; microbiology; Molecular Sequence Data; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; RNA, Ribosomal, 23S; genetics; RNA, Ribosomal, 5S; genetics; Ribotyping; Sequence Analysis, DNA
- From: Chinese Journal of Epidemiology 2003;24(12):1129-1131
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo define the main genotypes in Guizhou agricultural areas by molecular epidemiologic investigation of 21 Borrelia burgdorferi sensu lato of Lyme disease spirochetes and to provide the scientific bases for formulating a preventive policy.
METHODSPolymerase chain reaction (PCR) technique was used to amplify the 23S(rrl)-5S(rrf) intergenic spacer, and amplified products were analyzed by restriction fragment length polymorphism (RFLP) and nucleotide sequencing.
RESULTSThere were two genospecies in the strains: 20 strains belong to Borrelia valaisiana, 1 strain is Borelia sp.
CONCLUSIONBorrelia valaisiana was the main genotype in Guizhou agricultural areas. The harmness of B. valaisiana to human being has been confirmed. In order to efficiently prevent the harmness of agent to the people in Guizhou agriculture areas, we should study the risk further.
