A molecular-epidemiologic study on TEM-1 genes of Neisseria gonorrhoeae in Wuxi area.
- Author:
Zu-Huang MI
1
;
Ling QIN
Author Information
- Publication Type:Journal Article
- MeSH: China; epidemiology; Drug Resistance, Bacterial; genetics; Gonorrhea; epidemiology; microbiology; Humans; Neisseria gonorrhoeae; enzymology; genetics; Polymerase Chain Reaction; beta-Lactamases; genetics
- From: Chinese Journal of Epidemiology 2004;25(1):58-59
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn order to study the epidemiology of TEM-1 genes of Neisseria gonorrhoeae (Ng) in Wuxi area.
METHODSIn the light from foreign literature that positive Ng strains of beta-lactamase contain plasmid sequences of TEM-1 genes, heminested PCR for TME-1 genes of Ng detection was self-designed. Ng of 195 strains were detected, that were isolated in Wuxi area from Jan to the Oct, 2002.
RESULTSThere were 138 TEM-1 genes positive in 195 isolated strains with a positive rate of 70.8%. There was one case of heminested PCR product of TEM-1 genes which showed the homogeneity was 99% in Wuxi area, when comparing with pFA7 sequences of positive Ng plasmid of beta-lactase from register GenBank.
CONCLUSIONData showed that the Ng strains with TEM-1 genes were the prevalent ones in Wuxi area.
