Effects of long-term intervention of moxa smoke on T lymphocyte subsets and CD4+ CD25+ Treg in peripheral blood of Wistar rats.
- Author:
Ping LIU
1
;
Xiu-Jie PAN
;
Li HAN
;
Jia YANG
;
Hai HU
;
Hong CAI
;
Ruo-Xi LI
;
Mao-Xiang ZHU
;
Zhi-Hua YANG
;
Bai-Xiao ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Lymphocyte Count; Male; Moxibustion; Rats; Rats, Wistar; Smoke; analysis; T-Lymphocyte Subsets; drug effects; immunology; T-Lymphocytes, Regulatory; drug effects; immunology; Time Factors
- From: Chinese Acupuncture & Moxibustion 2013;33(2):145-148
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the cellular immune regulation of the long-term intervention of moxa smoke.
METHODSThirty-two Wistar rats were randomly divided into a blank group, a low concentration group, a medium concentration group and a high concentration group, 8 cases in each group. In addition to the blank group, rats in the other groups were exposed to the corresponding concentration moxa smoke for 20 min every day, the T lymphocyte subsets and proportion of the CD4+ CD25+ Treg in CD4+ T cells in peripheral blood were tested by flow cytometry after 6 months.
RESULTSCompared with the blank group, the proportions of CD3+ CD4+, CD3+ CD8+ T cells and CD3+ CD4/CD3+ CD8+ in the other 3 moxa smoke groups were not significantly different (P > 0.05), while the proportions of the CD4+ CD25+ Treg in CD4+ T cells were significantly lower (P < 0.05), but no statistically significant differences among those 3 moxa smoke intervention groups (P > 0.05).
CONCLUSIONLong-term moxa smoke intervention has no significant effect on the proportions of CD3+ CD4+, CD3+ CD8+ T cells and CD3+ CD4+/CD3+ CD8+, but it can decrease the proportions of the CD4+ CD25+ Treg in CD4+ T cells in peripheral blood of rats. The way produced by pretreatment with moxa smoke may play immunomodulatory effect.
