The study of combining high-risk human papillomavirus types checking and cytologic test in the screening of cervical lesions
10.3760/cma.j.issn.1003-9279.2011.04.020
- VernacularTitle:HPV高危型别检测联合细胞学检查对宫颈病变筛查的研究
- Author:
Xiao-Hong XU
1
;
Zheng-Xin XIE
;
Rong MA
;
Wei-Qin ZHANG
;
Qi-Fa LI
Author Information
1. 合肥市妇幼保健院
- Keywords:
Papilomavirus,human;
Cytology;
Polymerase chain reaction;
Viral load
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(4):298-300
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between thinprep cytologic test and the types of human papilloma virus (HPV) infection in cervical precancerous lesion screening. Methods To perform high-risk HPV types test in 1375 samples. Choose 256 positive samples to take thinprep cytologic test (TCT) and directed biopsies under colposcopy. Adopting two-channels real time PCR to genotype and quantify eight high risk HPV DNA (high risk types: HPV 16,18,45,31 ; intermediate risk types: HPV 33,52,58,67). Results There are 256 positive samples in High risk HPV DNA test ( 18. 62％ ). WNL rate for TCT is 16.41％ (42/256), ASCUS and above rate for TCT is 83.59％ (214/256). There is no statistically significant difference in the viral loads of HPV infection rate between the TCT negative patients and positive patients ( P ＞ 0. 5 ). Positive correspondence rate for TCT and biopsy are 92. 86％ ( 39/42),81.36％ (48/59) ,85.19％ (23/27)and 9/10. Conclusion High-risk HPV types checking combined with TCT and biopsy can raise positive rate significantly. It should be used as a reliable method for early diagnosis in cervical cancer and CIN screening.
