Establishment of cellular immunity of enhanced hepatitis B vaccine.
- Author:
Juan WANG
1
;
Gang WU
;
Li ZHAO
;
Ji-lai LI
;
Li-feng XU
;
Jing XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Enzyme-Linked Immunospot Assay; methods; Female; Hepatitis B; immunology; prevention & control; virology; Hepatitis B Surface Antigens; administration & dosage; immunology; Hepatitis B Vaccines; administration & dosage; immunology; Humans; Immunity, Cellular; Interferon-gamma; analysis; immunology; Mice; Mice, Inbred BALB C
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(4):310-312
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the method to detect the cellular immune response of enhanced hepatitis B vaccine and make verification preliminary.
METHODSImmunized BALB/c mice with enhanced hepatitis B vaccine and detected the IFN-gamma spots forming cells (SFC) of mouse spleen cell by Elispot. Optimized the conditions of the experiment. Cellular immune response between enhanced hepatitis B vaccine and normal hepatitis B vaccine by Elispot were compared.
RESULTSIFN-gamma SFC was higher in 5microg dose than in 2microg dose after immunization with enhanced hepatitis B vaccine and IFN-gamma SFC was declined after immunization 3 weeks ago. IFN-gamma SFC was higher in stimulus by peptide than by protein. Compared to normal hepatitis B vaccine, IFN-gamma SFC was higher in enhanced hepatitis B vaccine.
CONCLUSIONEstablished the detection method to evaluate the cellular immunity of enhanced hepatitis B vaccine and tested the repeatability.
