Establishment of cellular immunity of enhanced hepatitis B vaccine

VernacularTitle:免疫复合物型增效乙型肝炎疫苗细胞免疫检测方法的建立
Author: Juan WANG ¹ ; Gang WU ; Li ZHAO ; Ji-Lai LI ; Li-Feng XU ; Jing XU
Author Information

1. 北京生物制品研究所
Keywords: Hepatitis B vaccine; Enzyme tests
From: Chinese Journal of Experimental and Clinical Virology 2011;25(4):310-312
CountryChina
Language:Chinese
Abstract: Objective To establish the method to detect the cellular immune response of enhanced hepatitis B vaccine and make verification preliminary. Methods Immunized BALB/c mice with enhanced hepatitis B vaccine and detected the IFN-γ spots forming ceils (SFC) of mouse spleen cell by Elispot. Optimized the conditions of the experiment. Cellular immune response between enhanced hepatitis B vaccine and normal hepatitis B vaccine by Elispot were compared. Results IFN-γSFC was higher in 5μ g dose than in 2μg dose after immunization with enhanced hepatitis B vaccine and IFN-γ SFC was declined after immunization 3 weeks ago. IFN-γ SFC was higher in stimulus by peptide than by protein. Compared to normal hepatitis B vaccine, IFN-γ SFC was higher in enhanced hepatitis B vaccine. Conclusion Established the detection method to evaluate the cellular immunity of enhanced hepatitis B vaccine and tested the repeatability.