Alterations of NK cell frequency and function in HIV/HCV co-infected patients.
- Author:
Xin ZHANG
1
;
Peng ZHAO
;
Fu-biao KANG
;
Xin-wei ZHANG
;
Chan SI
;
Xue-li CHEN
;
Wei-wei CHEN
;
Min ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Case-Control Studies; Cell Count; Coinfection; immunology; Flow Cytometry; HIV Infections; immunology; Hepatitis C; immunology; Humans; K562 Cells; Killer Cells, Natural; cytology; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(4):248-250
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the impacts of HIV/HCV co-infection to NK cells by investigating the changes of frequencies and functions of NK cells in HIV/HCV co-infected patients.
METHODSFrequencies and counts of NK cells were measured in patients with HIV mono-infection, HCV monoinfection, HIV/HCV co-infection and health control (HC) group by flow cytometer (FCM). After stimulated with PMA and K562 cells, PBMCs were examined the proportion of IFN-gamma+ NK cells by FCM. Proportion of killed K562 cells were detected to analyze the killing functions of NK cells.
RESULTSThe frequencies of NK cells, the percentages of IFN-gamma+ NK cells as well as the functions of NK cells killing the K562 cells in HIV/HCV co-infection, HIV mono-infection and HCV mono-infection groups were all lower than those of HC group significantly, the absolute counts and killing functions of NK cells in co-infection group were significantly lower than those of HIV or HCV mono-infection group.
CONCLUSIONSThe counts and functions of NK cells were affected more in HIV/HCV co-infections than those in HIV or HCV mono-infection.
