The effect of extract of ginkgo biloba leaf during the formation of HBV-related hepatocellular carcinoma
10.3760/cma.j.issn.1003-9279.2011.05.003
- VernacularTitle:银杏叶提取物对HBV相关肝癌形成过程的影响
- Author:
Wei-Wei WANG
1
;
Jian-Chun GUO
;
Yun-Hao XUN
;
Li-Na XIAO
;
Wei-Zhen SHI
;
Jun-Ping SHI
;
Guo-Qiang LOU
Author Information
1. 杭州市第六人民医院
- Keywords:
Ginkgo biloba;
Hepatitis B virus;
Oncogenes;
Genes conversion;
Liver neoplosms;
Gemes,p53
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(5):325-327
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of extract of ginkgo biloba leaf(EGb) during the formation of HBV-related hepatocellular carcinoma ( HCC ).Methods 99 HBV transgenic mice were randomly divided into control group,high-dose group,low-dose group.High-dose group and low-dose group were intraperitoneal injected 35mg/(kg · d) and 17.5 mg/( kg · d) of the shuxuening injection.Control group without special treatment.The serological markers and immunohistochemical markers in liver tissue will be done at the first 12 months and 18 months.Results ( 1 ) HBV transgenic mice can be found HCC at the 18 months.The incidence of HCC was lower in high-dose group and low-dose group,there was statistically different among the three groups.(2) The semi-quantitative scoring of liver HBx expression was highest in the control group at the 12 months.The semi-quantitative scoring of liver HBx,p53 and Bcl-2 expression was highest in the control group at the 18 months.They all appeared statistically different among the three groups.(3) Spearman correlation analysis showed that HCC incidence and liver tissue HBx,p53,Bcl-2 expression was a certain degree of positive correlation.r was 0.536,0.487 and 0.403,P ＜ 0.05.Conclusion EGb can reduced the incidence of the HCC with HBV transgenic mice.The reason may be that the EGb can reduce liver HBx,p53,Bcl-2 protein expression in the HBV transgenic mice.
