Preliminary study of the Th17/Treg immunoregulation in patients coinfected with TB and HIV before and after HAART
10.3760/cma.j.issn.1003-9279.2011.05.010
- VernacularTitle:HIV/TB重叠感染者HAART治疗前后Th17/Treg免疫调节的初步研究
- Author:
Ying-Xia LIU
1
;
Gui-Lin YANG
;
Yang ZHOU
;
Hong-Mei ZHANG
;
Jie-Yun ZHANG
;
Si-Yuan WANG
;
Yan LIU
;
Shui-Teng LIU
;
Lu-Kun ZHANG
;
Hui WANG
Author Information
1. 深圳市第三人民医院
- Keywords:
CD4-positive T-Lymphocytes;
HIV;
Mycobeterium tuberculosis;
Infections
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(5):348-350
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the Th17/Treg (regulatory T cells) immunoregulation in patients coinfected with TB and HIV before and after HAART( highly active anti-retroviral therapy).Methods 10 HIV cases coinfected with TB ( HIV/TB group) and 10 cases infected with HIV only ( HIV group) received HAART.PBMCs were stained and immunophenotyping of Th17( IL-17 expressing T cells) and CD4 + CD25 +T cells (Treg) were analysed by flow cytometry.Results The pre-treatment patients tended to have lower Th17 cells and higher Tregs cells compared to post-treatment( 1.90％ ± 0.9％ vs.4.65％ ± 1.48％,16.48％ ±4.91％ vs.8.29％ ± 3.13％ respectively).The percentage of IL-17 before and after HAART were 1.90 ± 0.9％ vs.4.65 ± 1.48％ respectively in HIV/TB group patients ( P ＜ 0.01 ).The difference between the percentage of IL-17 before and after HAART in the HIV/TB group and the HIV group were 2.65 ± 1.62％ vs.0.67％ ± 0.46％ respectively (P ＜0.01 ).IL-17 expressing T cells were increased faster after HAART in the former group than the latter.The percentage of Treg before and after HAART were 16.48％ ±4.91％ vs.8.29％ ± 3.13％ respectively in HIV/TB group ( P ＜ 0.01 ).The difference between the percentage of Treg before and after HAART in the HIV/TB group and the HIV group were 8.91％ ±4.82％ vs.2.63％ ± 2.34％ respectively ( P ＜ 0.01 ).Treg were decreased more rapidly after HAART in the former than the latter.Conclusions TB and HAART both had an effect on the Th17/Treg ratio of HIV/ TB co-infected patients,which can cause increased Th17 expression,the later plays a pro-inflammatory role.TB and HAART can decrease Treg expression and enhance anti-inflammation response.The fact that Th17/Treg disorder are more likely to exist in patients with HIV/TB co-infection after HAART for one month suggests a potential role for Th17/Treg imbalance leading to tuberculosis-associated immune reconstitution inflammatory syndrome during patients receiving HAART period.
