Analysis of gag-specific IFN-gamma secretion responses in HIV-1 B/C recombinant infectors.
- Author:
Hong-Wei LIU
1
;
Kun-Xue HONG
;
Yuan YUAN
;
Chun-Hua LIU
;
Jian-Ping CHEN
;
Yu-Hua RUAN
;
Zhe WANG
;
Yi-Ming SHAO
Author Information
- Publication Type:Journal Article
- MeSH: CD4-Positive T-Lymphocytes; immunology; virology; Gene Products, gag; genetics; immunology; HIV Infections; genetics; immunology; virology; HIV-1; classification; genetics; immunology; Humans; Immunodominant Epitopes; Interferon-gamma; genetics; immunology; Recombination, Genetic
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(5):358-360
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo characterize the Gag-Specific T lymphocyte responses and identify immunodominant region recognized in Chinese HIV-1 recombinant subtype B/C infectors.
METHODS10 antiretroviral treatment (ART) naive HIV-1 recombinant subtype B/C infectors with infected time in 1 year, 25 ART-naive infectors with infected time > 3 years and 10 HIV-1-seronegative healthy individuals were enrolled. HIV-1-specific T lymphocyte responses were analyzed by an IFN-gamma Elispot assay against 123 overlapping peptides spanning HIV-1 Gag protein in the present study.
RESULTSGag-specific T lymphocyte responses of interferon-gamma secretion were identified in 8(8/10) Chinese HIV-1 recombinant subtype B/ C infectors with infected time in 1 year, the specific T lymphocytes are mainly targeted at five seperated peptides. Responses were identified in 17(68%) infectors with infected time more than 3 years, the specific T lymphocytes are mainly targeted at one peptide in p17 and six in p24. There was obviously positive correlation (P = 0.0318, r = 0.519) between the magnitude of responses and viremia in infectors infected time > 3 years. The magnitude of response in infectors infected in 1 year was significantly higher than group infected time > 3 years (P = 0.021). None of healthy individuals produced positive responses.
CONCLUSIONSHIV-1 recombinant subtype B/C Infectors at different stages of diseases recognize different region of gag.