Analysis of gag-specific IFN-γsecretion responses in HIV-1 B/C recombinant Infectors
10.3760/cma.j.issn.1003-9279.2011.05.013
- VernacularTitle:HIV-1B/C重组病毒感染者Gag特异性T淋巴细胞免疫应答的研究
- Author:
Hong-Wei LIU
1
;
Kun-Xue HONG
;
Yuan YUAN
;
Chun-Hua LIU
;
Jian-Ping CHEN
;
Yu-Hua RUAN
;
Zhe WANG
;
Yi-Ming SHAO
Author Information
1. 河南省疾病预防控制中心
- Keywords:
HIV;
Viral structural proteins;
T-Lymphocytes;
Enzyme tests;
Interferon type Ⅱ
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(5):358-360
- CountryChina
- Language:Chinese
-
Abstract:
Objective To characterize the Gag-Specific T lymphocyte responses and identify immunodominant region recognized in Chinese HIV-1 recombinant subtype B/C infectors.Methods 10 antiretroviral treatment(ART) na?ve HIV-1 recombinant subtype B/C infectors with infected time in 1 year,25 ART-na?ve infectors with infected time ＞ 3 years and 10 HIV-1-seronegative healthy individuals were enrolled.HIV-1-speeifie T lymphocyte responses were analyzed by an IFN-γ Elispot assay against 123 overlapping peptides spanning HIV-1 Gag protein in the present study.Results Gag-specific T lymphocyte responses of interferon-gamma secretion were identified in 8(8/10) Chinese HIV-1 recombinant subtype B/C infectors with infected time in 1 year,the specific T lymphocytes are mainly targeted at five seperated peptides.Responses were identified in 17 (68％) infectors with infected time more than 3 years,the specific T lymphocytes are mainly targeted at one peptide in p17 and six in p24.There was obviously positive correlation(P =0.0318,r =0.519 ) between the magnitude of responses and viremia in infectors infected time ＞ 3 years.The magnitude of response in infectors infected in 1 year was significantly higher than group infected time ＞ 3 years( P =0.021 ).None of healthy individuals produced positive responses.Conclusions HIV-1 recombinant subtype B/C Infectors at different stages of diseases recognize different region of gag.
