Construction of recombinant plasmid expressing S1 gene of new type of reovirus.
- Author:
Bing-Ke BAI
1
;
Wei-Zhi HUANG
;
Sheng-Dong LUO
;
Yan HU
;
Rong GAO
;
Zhi-Jie WANG
;
Qong HUANG
;
Hao-Dong LIU
;
Pan-Yong MAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cercopithecus aethiops; Gene Expression; Plasmids; genetics; metabolism; Recombinant Proteins; genetics; metabolism; Reoviridae; genetics; Vero Cells; Viral Proteins; genetics; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(5):361-363
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct the recombinant plasmid containing S1 gene of new type of reovirus, and to study the expression of protein sigma1 in Vero cells.
METHODSThe recombinant plasmid, named pC-S, was constructed by cloning S1 gene into vector pCAGGS/MCS. Then Vero cells were transfected with pC-S and collected at 24, 48, 72 h post transfection followed by SDS-PAGE and Western-Blot assay.
RESULTSResults both SDS-PAGE and Western-Blot assay indicated that sigma1 protein could be expressed well and the highest expression level was 72 h post transfection.
CONCLUSIONSSigma1 protein could be expressed well in Vero cells by transfected with recombinant plasmid containing S1 gene, and could give some implications for subsequent research on virus-host interactions.
