Construction of recombinant plasmid expressing S1 gene of new type of reovirus
10.3760/cma.j.issn.1003-9279.2011.05.014
- VernacularTitle:新型呼肠病毒S1基因真核表达质粒的构建及表达
- Author:
Bing-Ke BAI
1
;
Wei-Zhi HUANG
;
Sheng-Dong LUO
;
Yan HU
;
Rong GAO
;
Zhi-Jie WANG
;
Qong HUANG
;
Hao-Dong LIU
;
Pan-Yong MAO
Author Information
1. 解放军第302医院
- Keywords:
Reoviridae;
Gene expression regulation;
viral;
Viral proteins
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(5):361-363
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the recombinant plasmid containing S1 gene of new type of reovirus,and to study the expression of protein σ1 in Vero cells.Methods The recombinant plasmid,named pC-S,was constructed by cloning S1 gene into vector pCAGGS/MCS.Then Vero cells were transfected with pC-S and collected at 24,48,72 h post transfection followed by SDS-PAGE and Western-Blot assay.Results Results Both SDS-PAGE and Western-Blot assay indicated that σ1 protein could be expressed well and the highest expression level was 72 h post transfection.Conclusions σ1 protein could be expressed well in Vero cells by transfected with recombinant plasmid containing S1 gene,and could give some implications for subsequent research on virus-host interactions.
