Hypomethylation of TNF-alpha gene promoter in the patients with acute-on-chronic hepatitis B liver failure.
- Author:
Li-Yan HAN
1
;
Zhao-Yang GUO
;
Yu-Chen FAN
;
Kai WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; DNA Methylation; Female; Hepatitis B, Chronic; blood; genetics; metabolism; Humans; Liver Failure, Acute; blood; genetics; metabolism; Male; Middle Aged; Promoter Regions, Genetic; Tumor Necrosis Factor-alpha; blood; genetics; Young Adult
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(5):368-370
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe present study was designed to investigate the possible epigenetic alteration in the promoter of TNF-alpha in the patients with acute-on-chronic hepatitis B liver failure (ACHBLF).
METHODSThe methylation of TNF-alpha promoter in peripheral blood mononuclear cells (PBMCs) was measured by methylation specific PCR (MSP). The level of serum TNF-alpha was determined by enzyme-linked immunosorbent assay (ELISA). Model for End-stage Liver Disease (MELD) was performed for the evaluation of liver failure.
RESULTSThe serum level of TNF-alpha in patients with ACHBLF(44.9260 +/- 26.48523) was higher than that in CHB (18.92505 +/- 9.04461) and healthy controls (11.9172 +/- 5.04612) (P < 0.05). Moreover, the serum TNF-alpha level was significantly decreased in methylation group as compared to unmethylaiton group in patients with ACHBLF (P < 0.05). MELD was not significantly different between methylated and unmethylated group of ACHBLF patients (P > 0.05). In addition, the serum level of TNF-alpha was found to be positively correlated with serum total bilirubin (r = 0.891, P < 0.01) and MELD score (r = 0.792, P < 0.01), but to be negatively correlated with prothrombin activity (r = - 0.511, P < 0.05) in patients with ACHBLF.
CONCLUSIONThe TNF-alpha methylation patten is stable for the liver failure, suggesting the effect of environment on methylation.
