Hypomethylation of TNF-α gene promoter in the patients with acute-on-chronic hepatitis B liver failure
10.3760/cma.j.issn.1003-9279.2011.05.016
- VernacularTitle:慢加急性肝衰竭患者外周血肿瘤坏死因子-α基因启动子甲基化状态的研究
- Author:
Li-Yan HAN
1
;
Zhao-Yang GUO
;
Yu-Chen FAN
;
Kai WANG
Author Information
1. 山东大学齐鲁医院
- Keywords:
Liver failure;
Tumor necrosis factor;
DNA;
Polymerase chain reaction
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(5):368-370
- CountryChina
- Language:Chinese
-
Abstract:
Objective The present study was designed to investigate the possible epigenetic alteration in the promoter of TNF-α in the patients with acute-on-chronic hepatitis B liver failure (ACHBLF).Methods The methylation of TNF-α promoter in peripheral blood mononuclear cells (PBMCs) was measured by methylation specific PCR (MSP).The level of serum TNF-α was determined by enzyme-linked immunosorbent assay (ELISA).Model for End-stage Liver Disease (MELD) was performed for the evaluation of liver failure.Results The serum level of TNF-α in patients with ACHBLF(44.9260 ±26.48523 ) was higher than that in CHB ( 18.92505 ± 9.04461 ) and healthy controls ( 11.9172 ± 5.04612 )(P ＜ 0.05 ).Moreover,the serum TNF-α level was significantly decreased in methylation group as compared to unmethylaiton group in patients with ACHBLF(P ＜ 0.05).MELD was not significantly different between methylated and unmethylated group of ACHBLF patients (P ＞0.05 ).In addition,the serum level of TNF-αwas found to be positively correlated with serum total bilirubin ( r =0.891,P ＜ 0.01 ) and MELD score ( r =0.792,P ＜0.01 ),but to be negatively correlated with prothrombin activity ( r =- 0.511,P ＜ 0.05 ) in patients with ACHBLF.Conclusion The TNF-α methylation patten is stable for the liver failure,suggesting the effect of environment on methylation.
