Expression, purification and identification of St. Louis encephalitis virus-like particles in eukaryotic cells.
- Author:
Xue GUO
1
;
Fu-Bin XING
;
Qing-Jie XIAN
;
Lin WANG
;
Peng-Fei YANG
;
Li-Ping ZHANG
;
Rui-Jin PING
;
Kong-Xin HU
Author Information
- Publication Type:Journal Article
- MeSH: Encephalitis Virus, St. Louis; immunology; Encephalitis, St. Louis; diagnosis; Enzyme-Linked Immunosorbent Assay; HEK293 Cells; Humans; Plasmids; Recombinant Proteins; immunology; isolation & purification; Transfection; Virion; immunology; isolation & purification
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(6):405-408
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo express St. Louis encephalitis virus-like particles in mammalian cells, it will provide a prerequisite for further immunological diagnostic studies.
METHODS293T-cell were transiently transfected with recombinant PreM-E plasmid. Expression and antigenicity of the purified protein were determined by transmission electron microscope (TEM), Western-Blot, immunofluorescence assay and ELISA.
RESULTSRecombinant subviral particles, about 50 nm in diameter, were observed by TEM in the supernatant of transfected cells. The results of Western-Blot, IFA and ELISA showed the recombinant proteins retained immunoreactivity similar to those of native virus proteins.
CONCLUSIONSt. Louis encephalitis virus-like particles has good antigenicity and physical appearance. It will provide a prerequisite for further immune diagnostic reagent.
