Anti-adenovirus neutralizing antibodies and Gag-specific cellular immune responses in Macaca fascicularis immunized with Ad5-HIVgag
10.3760/cma.j.issn.1003-9279.2011.06.004
- VernacularTitle:Ad5-HIVgag免疫的食蟹猴中腺病毒中和抗体与Gag特异性细胞免疫反应的研究
- Author:
Yun LI
1
;
Liu YANG
;
Ling YANG
;
Dan-Ying CHEN
;
Xia FENG
;
Shuang-Qing YU
;
Ze-Lin LI
;
Yi ZENG
Author Information
1. 北京工业大学
- Keywords:
Adenoviridae;
HIV-1;
Macaca;
Immunity,cellular;
Neutralization tests
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(6):413-415
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the level of anti-adenovirus neutralizing antibodies and Gagspecific cellular immune responses in Macaca fascicularis immunized with different dosage of recombinant adenovirus vaccine Ad5-HIVgag by repeated intramuscular injection.Methods The Macaca fascicularis were randomly divided into four groups of 6.Different amount of the purified Ad5-HIVgag(0.99 × 1011 VP,4.94 × 1011 VP,24.68 × 1011 VP) or PBS were administered in 3 weeks interval and five times.The level of anti-adenovims neutralizing antibodies and Gag-specific cellular immune responses at different time points were detected by neutralization assay and Elispot assay respectively.Results High level of anti-adenovirus neutralizing antibodies could be detected in three groups immunized with Ad5-HIVgag at 3 weeks after first immunization.The neutralizing antibodies reached peak at 8 weeks after primary immunization,and declined slightly at late time.Significant HIV-1 Gag-specific cellular immune responses were detected in all Ad5-HIVgag immunized groups at 5 weeks post first immunization.The Gag-specific cellular immune responses declined at 12 weeks and then increased with time.Conclusion Anti-adenovirus neutralizing antibodies could be induced in Macaca fascicularis immunized with Ad5-HIVgag by repeated intramuscular injection.And the Gag-specific cellular immune responses tended to increase with the injection times.The presence of anti-adenovirus neutralizing antibodies induced by vaccination with adenovirus vectors in Ad5-naive animals did not further reduce Gag-specific cellular immune responses.
