Study on the effect of realgar nanoparticles anfi-adenovirus in vitro
10.3760/cma.j.issn.1003-9279.2011.06.005
- VernacularTitle:雄黄纳米微粒在细胞水平上抑制腺病毒复制的初步研究
- Author:
Miao CHENG
1
;
Cheng-Xiang WANG
;
Hui-Fang WANG
;
Bao-Di GOU
;
Zhen ZHU
;
Ming-Zhe WANG
;
Hong-Ri XU
;
Wen-Bo XU
Author Information
1. 北京中医药大学东直门医院
- Keywords:
Adenoviridae;
Realgar;
Nano-technology;
Dose-respmse relatioaship,drug
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(6):416-419
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study was to establish a model that adenovirus type 3 (HAdV-3) infected on Hep-2 cell in order to explore anti-adenovirus3 ( HAdV-3 ) effect of Chinese medicine realgar in vitro.Method Use high-energy ball milling with distilled water to prepare realgar nanoparticles.The concentration of nanometer realgar was tested by molybdenum blue staining method and realgar nanoparticles' particle size was tested on Nano Series.The techinique of cell culture with ribavirin as positive control was to observe anti-adenovirus effect through prevention,treatment and direct inactivation of three kinds of drug delivery.Result This drug was found to be a potential inhibitor of HAdV-3 in a concentration-dependent manner with the median toxic concentration ( TC50 ) of 0.649 μg/ml in Hep-2 Cell culture.The median inhibition concentration( IC50 ) was 0.255 μg/ml when drug was added before infection.The IC50 was 0.142 μg/ml when drug was added after virus infection,and it was 0.117 μg/ml as the drug was added after it mixed with virus.The therapeutic index ( TI ) was 2.55,4.57and 5.55 respectively.Conclusion The direct inactivation effect of realgar nanoparticles is the most evident in three drug deliveries manner with the same concentration in vitro.
