The study on high-resolution HLA and human cytomegalovirus (HCMV) viremia in bone marrow transplantation recipients
10.3760/cma.j.issn.1003-9279.2011.06.008
- VernacularTitle:HLA高分辨等位基因与骨髓移植受者HCMV抗原血症研究
- Author:
Ya-Dan MA
1
;
Min-Huan LI
;
Xue-Qin MENG
;
Ya-Ping HUANG
;
Jian-Hua HU
;
Xiao-Ming CHIN
;
Jun FAN
;
Wei-Hang MA
Author Information
1. 浙江大学医学院附属第一医院
- Keywords:
Cytomegalovirus;
Antigenemia;
High-resolution HLA;
Bone marrow transplantation
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(6):427-430
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the correlation between high-resolution HLA-A * 1101,HLA-A * 0201,HLA-A * 2402,HLA-B * 4001,HLA-DRB1 * 0901 with HCMV pp65 antigenemia after bone marrow transplantation (BMT) in China.Methods 48 recipients doing BMT during 2009.2-2010.10 were selected in my hospital; HCMV pp65 was detected by ELISA or immunohistochemical methods.The frequency of HLA-A * 1101,HLA-A * 0201,HLA-A * 2402,HLA-B * 4001,HLA-DRB1 * 0901 alleles were determined by Polymerase chain reaction-sequence based typing( PCR - SBT).Results ① The BMT recipients were HCMV pp65 antigenic positive( 100％ ) ; ② The positive rate of HLA-A * 1101,HLA-A * 0201,HLA-A *2402,HLA-B * 4001 showed no obvious difference between 12 lower antigenemia group and 36 higher antigenemia group,the positive rate: HLA-A * 1101 were 33.3％ (8/24)and 20.8％ (15/72),HLA-A * 0201 were 4.2％ (1/24) and 13.9％ ( 10/72),HLA-A * 2402 were 12.5％ (3/24) and 19.4％ ( 14/72),HLA-B * 4001 were 16.7％ (4/24) and 12.5％ ( 9/72 ) ; ③ HLA-DRB1 * 0901 positive rate in higher antigenemia group was higher than the lower ( P =0.048 ),the positive rate were 4.2％ ( 1/24 ) and 19.4％ (14/72) ;④ HLA-DRB1 * 0901 recipients were higher pp65 antigenemia than HLA-A * 2402 recipients (P=0.007) and HLA-A * 1101 recipients (P =0.028),HLA-A * 0201 recipients were higher pp65 antigenemia than HLA-A * 2402 ( P =0.02),the pp65 antigenemia showed no obvious difference among the rest of high-resolution HLA groups (P ＞ 0.05).Conclusion HLA-DRB1 * 0901 alleles might be correlated with BMT recipients happened higher pp65 antigenemia,HLA-A * 2402 alleles might be correlated with BMT recipients happened lower pp65 antigenemia.
