Study on vector-specific and foreign gene-speciflc immune responses induced by rAd5 and rAAV2/1 vaccines
10.3760/cma.j.issn.1003-9279.2011.06.009
- VernacularTitle:rAd5和rAAV2/1载体疫苗诱导载体特异性和外源基因特异性免疫反应的研究
- Author:
Liu YANG
1
;
Yun LI
;
Ling YANG
;
Ling-Fei ZHANG
;
Xia FENG
;
Shuang-Qing YU
;
Dan-Ying CHEN
;
Ze-Lin LI
;
Yi ZENG
Author Information
1. 北京工业大学
- Keywords:
Adenoviridae;
Parvoviridae;
Immunity,cellular;
Antibody farmation;
HIV-1
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(6):431-433
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the foreign gene-specific and vector-specific immune responses in BALB/c mice immunized with rAd5 or rAAV2/1 expressing the same gene.Methods BALB/c mice were immunized with rAd5-gag or rAAV2/1-gag once,HIV-1 Gag-specific and vector- specific cellular immune responses were analyzed by Elispot assay,HIV-1 P24-specifc IgG and vector-specific IgG were tested by ELISA assay.Results Mice immunized with rAd5-gag induced potent Gag-specific cellular immune responses and that were significantly higher than Ad5-specfic cellular responses,while rAAV2/1-gag elicited weak Gag-specific and AAV2/1-specific cellular responses.Both P24-specific and Ad5-specific IgG titers induced by rAd5-gag were high and in similar level.Higher level of P24-specific IgG was found in mice inoculated with rAAV2/1-gag than rAd5-gag.And the P24-specific IgG titers were higher than the vectorspecific IgG titers in mice immunized with rAAV2/1.Conclusion rAd5 could elicit strong foreign genespecific cellular and humoral immune responses,weak vector-specific cellular responses and strong vectorspecific antibodies,rAAV2/1 could induce potent foreign gene-specific antibodies that were much higher than vector-specific IgG,while both foreign gene-specific and vector-specific cellular responses were very low.
