Molecular characteristics of noroviruses causing outbreaks of acute gastroenteritis in Huzhou
10.3760/cma.j.issn.1003-9279.2011.06.011
- VernacularTitle:感染性腹泻疫情中诺如病毒的分子流行病学分析
- Author:
Xiao-Fang WU
1
;
Lei JI
;
De-Shun XU
;
Jian-Kang HAN
;
Yue-Hua SHEN
;
Li-Ping CHEN
;
Yun-Feng ZHA
;
Ren-Shu TANG
;
Wen-Ting YAO
Author Information
1. 浙江省湖州市疾病预防控制中心
- Keywords:
Norovirus;
Diarrhea;
Epidemiology,molecular;
Sequence analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(6):437-440
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the molecular characteristics of Noroviruses causing outbreaks of acute gastroenteritis in Huzhou.Methods From 2008 to 2010,total 119 fecal specimens collected from outbreaks of acute gastroenteritis were tcsted for Norovirus. Partial sequence of RNA dependent RNA polymerase(RdRp) of the positive samples were amplified by RT-PCR,then the PCR production were purified,sequenced and put into phylogenetic analysis. Results 50 of 119 specimens were positive for Norovirus by real-time RT-PCR.Out of those 50 Norovirus positive specimens,9 were Norovirus Genogroup Ⅰ (GI) positive,35 were Norovirus Genogroup Ⅱ (GⅡ)positive,6 was both Norovirus GⅠ and GⅡ positive.12 PCR products for RdRp were selected for further studies on sequencing.Phylogenetic analysis revealed that the 5 GⅠ norovirus isolates were belonged to genotype GⅠ/2 and GⅠ/3.Of the 7 GⅡ norovirus isolates,6 were belonged to genotype GⅡ/4,1 was belonged to genotype GⅡb.Conclusion Norovirus is a major cause of outbreaks of acute gastroenteritis in Huzhou and the epidemic strains of norovirus isolated from Huzhou had a high degree of genetic diversity.
