The associated research on the expression of RUNX3 mRNA and protein in hepatic cell carcinoma as well as the relationship with clinical pathological parameters
10.3760/cma.j.issn.1003-9279.2011.06.012
- VernacularTitle:肝癌组织RUNX3mRNA和蛋白表达及其与临床病理的相关性研究
- Author:
Yan-Hui LU
1
;
Rui-Dan ZHENG
;
Cheng-Run XU
;
Jie CHEN
;
Wei-Ping WU
;
Bi-Fen CHEN
;
Jian-Guo LI
;
Zhi-Chuan LIN
Author Information
1. 福建漳州解放军第175医院(厦门大学附属东南医院)
- Keywords:
RNA,messemger;
Protein RUNX3;
Liver neoplasms;
Reverse transcription polymerase chain reaction;
Immunohistochemistry
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(6):441-444
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of RUNX3 mRNA and protein in hepatic cell carcinoma (HCC)and surrounding normal tissue,to analyze the relationship between RUNX3 expression and clinical pathological parameters.Methods Reverse transcription polymerase chain reaction ( RT-PCR ) and immunohistochemistry(IHC) were performed to detect the expression of RUNX3 mRNA and protein in HCC and surrounding normal tissue respectively,and their relationship with clinical pathological parameters were analyzed.Results The relative expression value of RUNX3 mRNA found in 51 cases HCC was 0.4509 ± 0.0963,and that did in 51 cases surrounding normal tissue was 0.9147 ±0.0222.The difference of RUNX3 mRNA expression between two kinds of samples was statistically significant( t =33.6087,P ＜ 0.001 ).The positives rate of RUNX3 protein expression found in 51 cases HCC tissue was 49.02％ (25/51)and that did in 51 cases surrounding normal tissue was 82.35％ (42/51).The difference of RUNX3 protein expression between two kinds of samples was statistically significant ( x2 =12.5706,P ＜ 0.005 ).The difference of RUNX3 mRNA and protein expression in some clinical pathological parameters involving differentiation degree,invasion,cancer thrombus and diversion in liver were statistically significant (P ＜ 0.05 ).However that were not in another clinical pathological parameters involving gender,cancer diameter,cancer location as well as hemorrhage and necrosis of cancer,histotype ( P ＞ 0.05 ).Conclusion The expression of RUNX3 mRNA and protein in HCC were significantly lower than that in surrounding normal tissue. The lower expression of runx3 protein in the HCC probably plays an important role in the tumorigenesis and development of HCC.The RUNX3 gene may be an anti-oncogene of HCC.
