Genotyping and mutational analysis of occult hepatitis B virus infection in blood donors of Shaoxing.
- Author:
Lie-Yong SANG
1
;
Li-Qiang FU
;
Fang FANG
;
Pei-Fen ZHUANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Blood Donors; DNA Mutational Analysis; Female; Genotype; Hepatitis B; virology; Hepatitis B Surface Antigens; blood; Hepatitis B virus; classification; genetics; Humans; Male; Middle Aged; Phylogeny
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(6):470-473
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the molecular biological characteristics of occult hepatitis B virus (HBV) infected blood donors in Shaoxing.
METHODS8692 blood donors were screened using ELISA. The occult HBV infection was determined by DNA analysis among the HBsAg negative subjects. DNA sequencing and mutational analysis were further performed in the HBV DNA positive samples. The overall situation of occult HBV infection was hereby evaluated and the possible underlying mechanisms discussed.
RESULTSAmong the 8644 HBsAg negative subjects out of 8692 blood donors, 8 were HBV DNA positive. The occult HBV infection rate was 0.92 per thousand (8/8692). Among the 8 samples, 6 were genotype C (75%) and 2 genotype B (25%). In addition, a specific mutation in "a" epitope was observed in 7 OBI virus strains by amino acid analysis.
CONCLUSIONThere were occult HBV infected among blood donors in Shaoxing, which is probably associated with the gene mutation of HBV virus.
