Genotyping and mutational analysis of occult hepatitis B virus infection in blood donors of Shaoxing

VernacularTitle:绍兴市无偿献血者中隐匿性HBV感染的基因型别及突变类型分析
Author: Lie-Yong SANG ¹ ; Li-Qiang FU ; Fang FANG ; Pei-Fen ZHUANG
Author Information

1. 绍兴市中心血站
Keywords: Hepatitis B virus; Blood donors; Infections; Mutation
From: Chinese Journal of Experimental and Clinical Virology 2011;25(6):470-473
CountryChina
Language:Chinese
Abstract: Objective To assess the molecular biological characteristics of occult hepatitis B virus ( HBV ) infected blood donors in Shaoxing.Methods 8692 blood donors were screened using ELISA.The occult HBV infection was determined by DNA analysis among the HBsAg negative subjects.DNA sequencing and mutational analysis were further performed in the HBV DNA positive samples.The overall situation of occult HBV infection was hereby evaluated and the possible underlying mechanisms discussed.Results Among the 8644 HBsAg negative subjects out of 8692 blood donors,8 were HBV DNA positive.The occult HBV infection rate was 0.92‰(8/8692).Among the 8 samples,6 were genotype C (75％) and 2 genotype B (25％).In addition,a specific mutation in "a" epitope was observed in 7OBI virus strains by amino acid analysis.Conclusion There were occult HBV infected among blood donors in Shaoxing,which is probably associated with the gene mutation of HBV virus.