A study of immunoassay by using quantum dots to detect Hantavirus infection

VernacularTitle:汉坦病毒感染的量子点标记免疫检测研究
Author: Ya SHEN ¹ ; Jie WANG ; Long BA ; Ping-Ping YAO ; Xiao-Zhao DENG ; Chang-Jun WANG ; Han-Ping ZHU ; Yun ZHANG ; Zhan-Qiu YANG
Author Information

1. 南京医科大学
Keywords: Hantavirus; Fluores cence technology
From: Chinese Journal of Experimental and Clinical Virology 2011;25(6):486-488
CountryChina
Language:Chinese
Abstract: Objective To develop a new method to detect anti-Hantavirus IgG antibodies (HV IgG) based on quantum dots (QDs) and indirect immune technique. Methods The carbodiimide crosslinking method was used to couple protein G and goat antihuman IgG on the surface of water-solubility QDs.The coverglass covered HV antigen was used as carrier,and QDs-PG-IgG conjugates was used as labeled second antibody to detect the HV-IgG in the serum samples. The detecting conditions were optimized.Results The optimum reaction time,pH and goat antihuman IgG concentration for conjugating the QDs with goat antihuman IgG were 6.0,2h,and 20μg/ml,respectively.The optimum working dilution of QDs-PG-IgG conjugates was 1: 200.The detection limit of the serum samples was about 1:1280 dilution.Conclusion The method established in this study has been demonstrated to be a specific,sensitive,rapid test for detecting HV antibodies,laying the foundation of single molecule detection.The anti-fluorescence quenching ability of this method was significant improved.