Establishment of confirmatory test for HBsAb in serum of coexistence of hepatitis B surface antigen and antibodies to HBsAg
10.3760/cma.j.issn.1003-9279.2011.06.028
- VernacularTitle:HBsAg和HBsAb双阳性乙肝患者血清中HBsAb确认方法的建立
- Author:
Jia LIU
1
;
Lin CHEN
;
Jun XU
;
Jing-Xia GUO
;
YONG-JI SONG
;
Jing ZHAO
;
Ai-Xia LIU
;
Li-Hua YANG
;
Bo-An LI
;
Yuan-Li MAO
Author Information
1. 解放军第302医院
- Keywords:
Hepatitis B;
Hepatitis B surface antigems;
Hepatitis B antibodies
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(6):492-494
- CountryChina
- Language:Chinese
-
Abstract:
Objective Establish a kind of confirmation method based on ELISA,and use to verify authenticity of HBsAb + in HBsAg + HBsAb + serum,pick and get rid of the false masculine gender the result,and avoid the mistake diagnosis.Method Collect 60 pieces of serum whose thick degree of H BsAg at 1000 COI above tested by ECLIA as confirm serum,mixed the confirm serum of different dilution with HBsAb positive serum to screen and verify best thick degree of HBsAg.Collected 40 pieces of HBsAg + HBsAb +serum,ELISA tested the descend rate of HBsAb COI after neutralized with confirm serum in order to confirm authenticity of HBsAb + in pieces of HBsAg + HBsAb + serum.Result When thick degree of HBsAg is 2000 COI,the performance of neutralization to HBsAb is best.The ELISA confirmatory test is fully consistent with the ECLIA method with true positive of 37 pieces of HBsAg + HBsAb + serum while falsepositive of 3 pieces of serum.Conclusion The ELISA confirm method is a simple,accurate and low cost initial validation method.
