Molecular epideiological and clinical feature of human calicivirus and adenovirus among children with diarrhea less than 5 years old from 2010 to 2011 in Lanzhou, Gansu province.
- Author:
Yong-Xia WANG
1
;
Dan-Di LI
;
Yu JIN
;
Qing ZHANG
;
Hong WANG
;
Xiang-Yu KONG
;
Yu-Ning LI
;
Zhao-Jun DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae Infections; epidemiology; virology; Adenoviruses, Human; genetics; Caliciviridae; genetics; Caliciviridae Infections; epidemiology; virology; Child, Preschool; China; epidemiology; Diarrhea; epidemiology; virology; Female; Humans; Infant; Infant, Newborn; Male; Molecular Epidemiology; Time Factors
- From: Chinese Journal of Experimental and Clinical Virology 2012;26(1):8-10
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the clinical and molecular epidemiology characteristics of calicivirus and adenovirus in children for viral diarrhea in Lanzhou.
METHODSStool samples were collected from 295 children with diarrhea at the First Hospital of Lanzhou University, Gansu Province,China, between July 2010 and June 2011. Reverse transcription-polymerase chain reaction (RT-PCR) or PCR were used to detected calicivirus and adenovirus. The adenovirus positive samples were typed by nested PCR and multiple PCR.
RESULTSOf the 295 specimens, 13.2% (39/295) were positive for calicivirus, and 5.1% (15/295) were adenovirus. Typing and Phylogenetic analysis revealed that novirus GII-3 and adenovirus 41 were the dominant strains. Both calicivirus and adenovirus predominately affect children under the age of 2. In seasonal distribution, there was no obvious peak.
CONCLUSIONHuman calicivirus and adenovirus are important pathogens of viral diarrhea,it is important to develop long-term systematic surveillance.
