Molecular epideiological and clinical feature of human calicivirus and adenovirus among children with diarrhea less than 5 years old from 2010 to 2011 in Lanzhou, Gansu province

VernacularTitle:2010-2011年兰州地区5岁以下腹泻患儿杯状病毒和腺病毒感染的流行特征
Author: Yong-Xia WANG ¹ ; Dan-Di LI ; Yu JIN ; Qing ZHANC ; Hong WANG ; Xiang-Yu KONG ; Yu-Ning LI ; Zhao-Jun DUAN
Author Information

1. 兰州大学第一医院
Keywords: Caliciviridae; Adenoviridae; Diarrhea
From: Chinese Journal of Experimental and Clinical Virology 2012;26(1):8-10
CountryChina
Language:Chinese
Abstract: Objective To investigate the clinical and molecular epidemiology characteristics of calicivirus and adenovirus in children for viral diarrhea in Lanzhou.Methods Stool samples were collected from 295 children with diarrhea at the First Hospital of Lanzhou University,Gansu Province,China,between July 2010 and June 2011.Reverse transcription-polymerase chain reaction (RT-PCR) or PCR were used to detected calicivirus and adenovirus.The adenovirus positive samples were typed by nested PCR and multiple PCR.Results Of the 295 specimens,13.2％ ( 39/295 ) were positive for calicivirus,and 5.1％ ( 15/295 ) were adenovirus.Typing and Phylogenetic analysis revealed that novirus GⅡ-3 and adenovirus 41 were the dominant strains.Both calicivirus and adenovirus predominately affect children under the age of 2.In seasonal distribution,there was no obvious peak.Conclusion Human calicivirus and adenovirus are important pathogens of viral diarrhea,it is important to develop long-term systematic surveillance.