Detection and typing assay of norovirus in acute hospitalizations among children less than 5 years old from 2008 to 2009 in Lulong, Hebei province
10.3760/cma.j.issn.1003-9279.2012.01.005
- VernacularTitle:河北省卢龙地区2008-2009年5岁以下腹泻住院儿童中诺如病毒的检测和分型
- Author:
Qiang LIN
1
;
Miao JIN
;
Hui-Ying LI
;
Shu-Xian CUI
;
Qing ZHANG
;
Na LIU
;
Shao-Long ENG
;
Zhao-Jun DUAN
Author Information
1. 南华大学
- Keywords:
Norovirus;
Gastroenteritis;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(1):11-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular epidemiologic characteristics and genotypes of norovirus in children less than 5 years of age in Lulong area from 2008 to 2009.Methods 325 stool specimens and epidemiological data from hospitalized children with diarrhea less than 5 years of age were collected.Rotavirus was detected by using the ELISA kit.Norovirus,adenovirus and astrovirus were detected by multiple reverse transcription-polymerase chain reaction (RT-PCR).Partial norovirus strains were sequenced and the tree was conducted by using the phylogenetic analyses.Results Norovirus was detected in 37out of 325 (11.3％ ) specimens,ranked only second to rotavirus (48.6％),and higher than adenovirus (6.5 ％ ) and astrovirus (4.3％).Norovirus predominantly infected children less than 2 years of age and the season peak of norovirus occurred in November.Phylogenetic analysis showed that the predominant strain was the GⅡ.4/2006b variant.Interestingly,a novel unreported GⅡ-4 variant was found in this study.Conclusion Norovirus was one of the most important pathogens causing acute gastroenteritis from 2008 to 2009 in Lulong area.The GⅡ.4/2006b vairant was still the predominant strain.It is important to keep on monitoring the novel GⅡ.4 variant.
