Molecular and epidemiological study on among children under 5 years old in Nanjing

VernacularTitle:南京地区婴幼儿杯状病毒感染的分子流行病学研究
Author: Xiao-Le LI ¹ ; Dan-Di LI ; Wei-Xia CHENG ; Guang-Cheng XIE ; Xiao-Qian GAO ; Gui-Ping KONG ; Yu JIN ; Zhao-Jun DUAN
Author Information

1. 南京大学医学院
Keywords: Caliciviridae; Diarrhea; Epidemiology,molecular
From: Chinese Journal of Experimental and Clinical Virology 2012;26(1):14-17
CountryChina
Language:Chinese
Abstract: Objective To study the infected information,clinical symptom and molecular epidemiological characteristics of HuCV infection among children under 5 years old in Nanjing.Methods In Nanjing Children's Hospital of Nanjing Medical University from July 2010 to June 2011,we collected 428 stool specimens from children with diarrhea and 428 asymptomatic controls.Human Calicivirus were tested by using RT-PCR.Then we sequenced the nucleic acid of PCR amplifications and identified the genotype and gene group of prevalent strains.Results 63 ( 14.72％ ) out of 428 stool samples were detected as HuCV.58 were norovirus and 5 were sapovirus,while G Ⅱ -4 2006b was the predominant strain of NoV.In the 428 control samples,19 samples were positive for calicivirus,there were 8 NoV and 13 SaV (Including 3 coinfection cases).Conclusion Human caliciviruses with different genotypes circulated among children in Nanjing,and G Ⅱ.2006b is the dominant genotype.