Optimizing expression of the capsid protein VP2 from human Bocavirus and establish it's seroepidemiology assying methord
10.3760/cma.j.issn.1003-9279.2012.01.007
- VernacularTitle:人博卡病毒VP2蛋白的原核表达及血清学检测方法的建立
- Author:
Ye-Xia HAO
1
;
Ji-Min GAO
;
Yu JIN
;
Xiao-Le LI
;
LIJing-song
;
Zhi-Ping XIE
;
Yuan-Yun AO
;
Xi-Qian CHEN
;
Ke-Na CHEN
;
Zhao-Jun DUAN
Author Information
1. 温州医学院
- Keywords:
Human Bocavirus;
Codon;
Expressied sequence tags;
Epidemiology,melecular;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(1):18-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain sufficient recombinant VP2 protein of human Bocavirus and establish it's seroepidemiology assying methord.Methord The capsid protein VP2 DNA genes of HBoV1 and 2 were optimized in accordance with the usage of the favorite codons in E.coil so as to enhance its protein expression in prokaryotic expressing system.The protein was purified by Ni-NTA column,and its antigenicity was determined by Western Blot.Then establish ELISA to detect the specific anti-VP2 IgG antibodies against HBoV1 and 2 in healthy children aged 3 - 6 years in Nanjing,China.Results The recombinant protein 6 × His-VP2 was produced in a larger quantity at 25℃ induced by IPTG (lmmol/L) over night and purified by Ni-NTA column.Seropositive rates of HBoV1and 2 were 62.2％ and 55.5％ and their mixed seropositivity was 37％.Conclusion The optimizing expression of the capsid protein VP2 from human Bocavirus constructed successfully and get a high yield under certain conditions.The established ELISA could be used to further analyze seroepidemiology of HBoV in china.
