Hepatitis B virus (HBV) mutation in Enhancer Ⅰ(HBV Enh Ⅰ) / X-promoter and the relationship between chronic HBV-related disease spectrum
10.3760/cma.j.issn.1003-9279.2012.01.009
- VernacularTitle:乙型肝炎病毒增强子Ⅰ/X基因启动子变异与HBV慢性感染疾病谱的关系
- Author:
Hao-Hui DENG
1
;
Yu-Juan GUAN
;
Chun-Liang LEI
;
Min XU
;
Feng-Yu HU
;
Jie CHENG
;
Yue-Ping LI
Author Information
1. 广州市第八人民医院
- Keywords:
Hepatitis B virus;
Enhancer elements(genetics);
Mutation
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(1):25-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the hepatitis B virus (HBV) mutation in the Enhancer Ⅰ (HBV Enh Ⅰ ) / X-promoter and to analysis the relationship between chronic HBV-related disease spectrum.Methods 275 patients were enrolled in this study,including 100 cases of chronic hepatitis B (CHB),74 cases of liver cirrhosis (LC),101 cases of hepatocellular carcinoma (HCC),grouping by different HBV genotypes,using semi-nested PCR amplification of HBV Enh Ⅰ / X-promoter and sequencing DNA,the mutations were determined by alignment to HBV reference sequence,the data was compared by x2 test and analyzed by multivariate logistic regression.Results ①Genotyping results:61.48％ (158/257) were infected with HBV genotype B,including 70 cases of CHB,36 cases of LC and 52 cases of HCC;38.52％ (117/257 ) were infected with HBV genotype C,including 30 cases of CHB,38 cases of LC and 49 cases of HCC. ②In the patients were infected with HBV genotype B,A1123Y mutation in LC was significantly higher than in CHB (30.56％ vs.8.58％,x2 =8.533,P =0.005,A =4.693,95％ CI[ 1.567 -14.056 ] ),HCC was significantly higher than in CHB (28.85％ vs.8.58％,x2 =8.607,P =0.003,A =4.324,95％ CI [ 1.544 -12.109]);A1317G mutation in HCC was significantly higher than in CHB (30.77％ vs.7.14％,x2 =11.687,P =0.001,A =5.778,95％ CI[ 1.955 - 17.076] ).In the patients were infected with HBV genotype C,T1323C mutation in HCC was significantly higher than in C HB (30.61％ vs.6.67％,x2 =6.318,P =0.012,A =6.176,95％ CI [ 1.301 - 29.331 ] ).③ Multivariate regression analyses showed that A1317G(OR =5.706,95％ CI[1.770 - 18.837],P =0.004) and T1323C (A =5.810,95％ CI[ 1.114 - 30.306],P =0.037) mutation were risk factors for HCC.Conclusion HBV Enh Ⅰ / X-promoter mutations were associated with the development of LC and HCC,the mutations can help to predict the occurrence of LC and HCC.
