Comparative study on the HPV infection rate of different esophageal squamous cell carcinoma in Anyang China.
- Author:
Peng QU
1
;
Jin-Tao LI
;
Li-Dong WANG
;
Yi ZENG
;
Xiu-Sheng CHU
Author Information
- Publication Type:Journal Article
- MeSH: Capsid Proteins; analysis; Carcinoma, Squamous Cell; virology; DNA, Viral; isolation & purification; Esophageal Neoplasms; virology; Human papillomavirus 16; isolation & purification; Human papillomavirus 18; isolation & purification; Humans; Oncogene Proteins, Viral; analysis; Papillomaviridae; isolation & purification; Papillomavirus Infections; epidemiology; Polymerase Chain Reaction
- From: Chinese Journal of Experimental and Clinical Virology 2012;26(1):34-36
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEComparative and statistical analysis the HPV infection rate between fresh tissue and Paraffin-embedded Specimens of esophageal squamous cell carcinoma,and comparative the testing results with others regions.
METHODSExtracted the total DNA from the novel fresh tissue and Paraffin-embedded Specimens; Detected the DNA by PCR with universal primer and Detected the HPV type with human papilloma virus nucleic acid amplification-based typing detection reagent kit (Hybribio); Compared the statistical result from the different specimens; analyzed the result between different region.
RESULTSHPV infection rate of fresh tissue is 82.6% with HPV16 (34.8%) and HPV18 (34.8%), and paraffin-embedded specimens is 78.2% with HPV16 (30.4%) and HPV18 (17.4%).
CONCLUSIONThe results provides the first evidence that there wasn't noticeable difference between HPV infection rate of the two specimens. So broader specimen source could be used for HPV testing.
